Last edited by Fezragore
Friday, May 8, 2020 | History

2 edition of Protein interaction studies on the rotavirus non-structural protein NSP1 found in the catalog.

Protein interaction studies on the rotavirus non-structural protein NSP1

Catherine Isabelle Thompson

Protein interaction studies on the rotavirus non-structural protein NSP1

by Catherine Isabelle Thompson

  • 2 Want to read
  • 6 Currently reading

Published by typescript in [s.l.] .
Written in English


Edition Notes

Thesis (Ph.D.) - University of Warwick, 1999.

StatementCatherine Isabelle Thompson.
The Physical Object
Paginationxv, [282]p.
Number of Pages282
ID Numbers
Open LibraryOL21338594M

non-structural proteins (NSP). Cleavage of VP4 leads to VP5* involved in cell penetration, and VP8*, the head of the VP4 spike required for attachment and infection of intestinal cells,15 Infection with naturally-occurring Rotavirus strains does not confer Cited by: 1. Rotavirus is a major cause of acute infantile diarrhoea worldwide. The virus genome consists of 11 segments of double-stranded RNA that codes for six structural proteins (VP) and six non-structural proteins (NSP1 6). NSPs are proteins expressed from the virus genome in the infected cell, but are not incorporated into the mature virus.

Rotavirus remains a major cause of diarrhea in infants and young children worldwide. The permanent emergence of new genotypes puts the potential effectiveness of vaccines under serious question. The distribution of unusual genotypes subject to viral fitness is influenced by interactions among viral proteins. The present work aimed at analyzing the genetic constellation and the coevolution of Author: Nabil Abid, Giovanni Chillemi, Marco Salemi. I found that RV-encoded non-structural protein 1 (NSP1) hijacks the host cullin-3 E3 ligase complex to target β-TrCP for co-destruction at the proteasome, thereby inhibiting the NF-κB signaling, challenging the current paradigm that NSP1 itself is a viral E3 ligase.

Three monkey kidney cell lines, Vero, GL-V3 and MA were subjected to karyological analysis to determine their chromosomal stability and to confirm their species of origin. Although the lines were shown to be relatively stable throughout all of the passage levels that were tested, the species of origin of one of them was found to be different from that claimed by the originators. Transcription of the rotavirus genome segments results in the synthesis of mRNA that can encode six structural proteins (mentioned above) that will be used for assembly of new progeny virions, and six non-structural proteins that encode a variety of functions to enable virus takeover of the host cell machinery (NSP1 to NSP6).


Share this book
You might also like
Pearls on a string

Pearls on a string

fossil salamanders of the family Sirenidae

fossil salamanders of the family Sirenidae

Physical education and health education for Wyoming elementary schools

Physical education and health education for Wyoming elementary schools

Dukes Diamonds

Dukes Diamonds

Outline of the general report upon the size of farms, and upon the persons who cultivate farms.

Outline of the general report upon the size of farms, and upon the persons who cultivate farms.

Legal and economic institutions for private sector growth in post-conflict economies

Legal and economic institutions for private sector growth in post-conflict economies

Hospital design and the National Health Service

Hospital design and the National Health Service

Civil architecture, or, A complete theoretical and practical system of building ...

Civil architecture, or, A complete theoretical and practical system of building ...

Print of full town report.

Print of full town report.

national union catalog

national union catalog

Intermediate GNVQ business

Intermediate GNVQ business

The Photosystems

The Photosystems

Sedimentary petrography

Sedimentary petrography

Protein interaction studies on the rotavirus non-structural protein NSP1 by Catherine Isabelle Thompson Download PDF EPUB FB2

Thompson, Catherine Isabelle () Protein interaction studies on the rotavirus non-structural protein NSP1. PhD thesis, University of : Catherine Isabelle Thompson. Protein-protein interactions between NSP1 and VP1, VP2, VP3, and NSP3, from the UKtc strain of bovine rotavirus, were investigated using a variety of approaches, the first of which was the yeast two-hybrid system.

In this assay a self-interaction of NSP1 was not : Catherine Isabelle Thompson. Protein interaction studies on the rotavirus non-structural protein NSP1. (Thesis) Thompson CI. Publisher: University of Warwick [] Metadata Source: The British Library Type: Thesis.

Abstract. Highlight Terms No biological terms identified. behavior of rotavirus under these conditions is consistent with the idea that the virus encodes proteins that suppress the activation of ISGs or that inhibit the activity of the ISG products.

The recently identified interaction between the rotavirus non-structural protein 1 (NSP1) and IRF3 by using a yeast two-hybridCited by: The rotavirus genome consists of 11 double-stranded (ds)RNA segments, and each segment encodes one protein with the exception of segm which in some rotavirus strains codes for 2 proteins, NSP5 and NSP6 [1, 2].

Of these proteins encoded by the viral genome, six are structural (VPs) and the remaining are non-structural (NSPs).Cited by: The rotavirus genome consists of 11 double-stranded (ds)RNA segments, and each segment encodes one protein with the exception of segm which in some rotavirus strains codes for 2 proteins, NSP5 and NSP6 [1,2].

Of these proteins encoded by the viral genome, six are structural (VPs) and the remaining are non-structural (NSPs).Cited by: The recently identified interaction between the rotavirus non-structural protein 1 (NSP1) and IRF3 by using a yeast two-hybrid system suggests that NSP1 may be involved in modulating the innate immune response (15).

NSP1, the product of the rotavirus gene 5 RNA, is a kDa RNA-binding protein that accumulates in the cytoplasm of the infected cell. Non-structural protein 1 (NSP1) of RV is a 55 KD protein which plays a vital role in antagonizing the IFN immune response.

NSP1 is also found to activate PI3K/AKT mediated anti-apoptotic pathway [27] through its ability to bind p85 subunit of PI3K for activation of AKT [28], resulting in efficient virus infection and by: The nonstructural protein 1 (NSP1) of rotavirus is a well-studied viral protein that interacts with various host proteins and targets them for proteasomal degradation [].

NSP1, the product of rotavirus gene 5, is a nonstructural RNA-binding protein that contains a cysteine-rich region and is a component of early replication intermediates. RNA- folding predictions suggest that this region of the NSP1 mRNA can interact with itself, producing a stem-loop structure similar to that found near the 5'-terminus of the NSP1 ro: IPR The highly flexible VP4 spike protein on rotavirus assumes altered conformations due to proteolytic cleavage or encountering high pH.

a Rotavirus grown in the absence of trypsin (upper panel) has. The rotavirus genome encodes six nonstructural (NS) proteins, five of which (NSP1, NSP2, NSP3, NSP5, and NSP6) have been suggested to be involved in a variety of events, such as genome replication, regulation of gene expression, and gene assortment.

These NS proteins have been found to be associated with replication complexes that are precursors of the viral core, however, little Cited by: Plays a role in the inhibition of host innate immunity by inducing the degradation of key host factors required to activate interferon production such as IRF3, IRF5 or IRF7.

Associates with components of cullin RING ligases (CRLs) including CUL1 or CUL3, which are essential multisubunit ubiquitination complexes, to modulate their activities.

The role of the rotavirus non-structural proteins NSP1 and NSP3 in regulating cellular and viral mRNA translation has been investigated by examining the effect of added recombinant NSP3 on protein translation in a T7-based in vitro coupled transcription-translation system.

Addition of purified NSP3 to assays primed solely with cellular mRNA was found to have no effect on the translation Cited by: 5. Introduction. The rotavirus genome consists of 11 double-stranded (ds)RNA segments, and each segment encodes one protein with the exception of segm which in some rotavirus strains codes for 2 proteins, NSP5 and NSP6 [1,2].Of these proteins encoded by the viral genome, six are structural (VPs) and the remaining are non-structural (NSPs).

In human cells in vitro, evasion of the innate IFN response is mediated principally by rotavirus non-structural protein 1 (NSP1) through inhibition of NF-κB activation via degradation of F-box/WD. Rotavirus (RV) contains 11 double-stranded RNA segments that encode for twelve structural and nonstructural proteins.

The separation and isolation of viral RNA is a necessary precursor for many experimental techniques and can be useful for rapid RV RNA typing and sequencing of different rotavirus strains. Non-structural RNA-binding protein 53 UniRule annotation Short name: NS53 UniRule annotation Non-structural protein 1 Add BLAST: View protein in InterPro IPR Rotavirus_NSP1: Pfam i: View protein in Pfam PF Rota_NS53, 1 hit.

The 12 kDa non-structural protein 6 (NSP6) is the least studied of the rotavirus proteins. In an attempt to further characterize this protein mono-specific antisera was generated using purified protein expressed in E.

radio-labeling of virus infected cells was used to show that it is expressed at a steady but low rate throughout the virus replication by: In this study, we show that rotavirus NSP1 targets TRAF2 to regulate IFN induced non-canonical NFκB activation.

Here we found that rotavirus Non-Structural Protein-1 (NSP1) interacts with TRAF2 and degrades it in a proteasome dependent manner. C-terminal part of NSP1 was sufficient for interacting with TRAF2 but it alone could not degrade TRAF2. Author summary Chikungunya virus (CHIKV) is an emerging threat to world health.

It is transmitted by Aedes species mosquitos, and has caused massive epidemics across the globe. The virus causes fever, rash, arthritis and can sometimes be fatal. The biology of CHIKV is poorly understood, to address this deficiency we aimed to identify functions of one of the viral proteins, by: 4.

Bluetongue virus (BTV) 1 is a representative member of the Orbivirus genus within the Reoviridae family and has a tensegment double-stranded RNA (dsRNA) genome enclosed within a double capsid.

The segments code for seven capsid and viral core proteins (VP1–VP7). The remaining three segments encode non-structural proteins (NS1, NS2, and NS3/NS3A) that are produced in the.

The product of the rotavirus g5(+) RNA is NSP1, a nonstructural protein whose interaction with the cytoplasmic resident protein, interferon (IFN) regulatory factor 3 (IRF3) (Graff et al, ), promotes cell‐to‐cell spread of the virus (Barro and Patton, ).Cited by: